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Open Access Research article

Pinewood nematode-associated bacteria contribute to oxidative stress resistance of Bursaphelenchus xylophilus

Cláudia S L Vicente12, Yoriko Ikuyo1, Manuel Mota23 and Koichi Hasegawa1*

Author Affiliations

1 Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan

2 ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, Évora 7002-554, Portugal

3 INIAV/Unidade Estratégica de Investigação e Serviços de Sistemas Agrários e Florestais e Sanidade Vegetal Av. da República, Quinta do Marquês, Oeiras 2784-159, Portugal

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BMC Microbiology 2013, 13:299  doi:10.1186/1471-2180-13-299

Published: 23 December 2013

Abstract

Background

Pine wilt disease (PWD) caused by the pinewood nematode Bursaphelenchus xylophilus is one of the most serious forest diseases in the world. The role of B. xylophilus-associated bacteria in PWD and their interaction with the nematode, have recently been under substantial investigation. Several studies report a potential contribution of the bacteria for the PWD development, either as a helper to enhance the pathogenicity of the nematode or as a pathogenic agent expressing interesting traits related to lifestyle host-adaptation.

Results

We investigated the nematode-bacteria interaction under a severe oxidative stress (OS) condition using a pro-oxidant hydrogen peroxide and explored the adhesion ability of these bacteria to the cuticle surface of the nematodes. Our results clearly demonstrated a beneficial effect of the Serratia spp. (isolates LCN-4, LCN-16 and PWN-146) to B. xylophilus under the OS condition. Serratia spp. was found to be extremely OS-resistant, and promote survival of B. xylophilus and down-regulate two B. xylophilus catalase genes (Bxy-ctl-1 and Bxy-ctl-2). In addition, we show that the virulent isolate (Ka4) of B. xylophilus survives better than the avirulent (C14-5) isolate under the OS condition. The bacterial effect was transverse for both B. xylophilus isolates. We could not observe a strong and specific adhesion of these bacteria on the B. xylophilus cuticle surface.

Conclusions

We report, for the first time, that B. xylophilus associated bacteria may assist the nematode opportunistically in the disease, and that a virulent B. xylophilus isolate displayed a higher tolerance towards the OS conditions than an avirulent isolate.

Keywords:
Bursaphelenchus xylophilus; Catalase; Oxidative stress; Pine wilt disease