Cytokine production by DCs conditioned with culture medium from MODE-K cells ± L. gasseri OLL2809/L13-Ia. iDCs were challenged for 24 h with the same media described in Figure 4 and then LPS pulsed. IL-12, TNF-α and IL-10 levels were detected in the supernatants of DC cultures by sandwich-type ELISA; values are expressed in pg/ml; columns represent the mean ± SD and are representative of three independent experiments. SupMODE supernatant from 24 h culture of MODE-K cells; SupOLL2809 and SupL13-Ia, supernatants from irradiated bacteria incubated for 24 h in RPMI complete medium. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Luongo et al. BMC Microbiology 2013 13:298 doi:10.1186/1471-2180-13-298