Table 2

Oligonucleotide primers
Oligonucleotide primers Sequence (designed restriction sites are underlined)
PhaP1 NdeI 5′-GGAATTCCATATGATGTTCAAGGTTGAAGACTT-3′
PhaP1 XhoI 5′-CCGCTCGAGTTAGTTGGCGGCCGGGGTGA-3′
PhaP2 NdeI 5′-GGAATTCCATATGGTGAGTGATGCCGATATGAA-3′
PhaP2 XhoI 5′-CCGCTCGAGTCAAGCAGCCCTATGCAGAA-3′
PhaP3 NdeI 5′-GGAATTCCATATGATGATCGAACCGAAAATCGA-3′
PhaP3 XhoI 5′-CCGCTCGAGTCAGGATTTGCCCTTGCCCG-3′
PhaP4 NdeI 5′-GGAATTCCATATGATGACAGGTGCGACTGATCC-3′
PhaP4 HindIII 5′-CCCAAGCTTTCAGGCGGCGGGCGGGTAGA-3′
PhaR NdeI 5′-GGAATTCCATATGGCGAAATCAGACCAACCCAC-3′
PhaR HindIII 5′-CCCAAGCTTCTACTCTTCCTTCTTCGACA-3′
RTphbA1-F 5′-CATCGCCGTCAACAAGGA-3′
RTphbA1-R 5′-CCGCTTCTGCATCTCGAAC-3′
RTphbA2-F 5′-AAGAAGGCCGGCTGGAA-3′
RTphbA2-R 5′-CCATTGACGTTGACCTTGGA-3′
RTphbB1-F 5′-TCGAACTACGACGCCTGTG-3′
RTphbB1-R 5′-ATGCCGTCCTTGGTGATG-3′
RTphbB2-F 5′-CCGAAGGCGTGAAGAAGGT-3′
RTphbB2-R 5′-GAACAGCGAGCCGAGATTG-3′
RTphbC1-F 5′-GCTCTGGGAAAACATCTGGAAC-3′
RTphbC1-R 5′-TTGGTGATGGTGCGGAAA-3′
RTphbC2-F 5′-GGACGACTACGTTGAGGATGG-3′
RTphbC2-R 5′-AATGGCGAGTGCGGATG-3′
RTphbC3-F 5′-ATGACCGCGTCGAACCA-3′
RTphbC3-R 5′-GGCACCTTGACCTTGGAGA-3′
RTphbC4-F 5′-GGCGAAGACAGGCAAACA-3′
RTphbC4-R 5′-CTCCATCCATCCGAACCA-3′
RTphbC5-F 5′-CCGCAAAATTCCCTGGTC-3′
RTphbC5-R 5′-CATCCCTGTCCTTCGCATC-3′
RTphaZ1-F 5′-CCGAAGCAACGCACACA-3′
RTphaZ1-R 5′-ATCCTCGGCACGATTTCC-3′
RTphaZ2-F 5′-GGCACATCAAGCAGCACA-3′
RTphaZ2-R 5′-AGATCCATCACCGCGAAA-3′
RTphaP1-F 5′-ACGGCGACTACACCAAGAAG-3′
RTphaP1-R 5′-GAAGGTCTCGTAGGCGGAAC-3′
RTphaP2-F 5′-TCGCTTTTACCGAGCAGAAC-3′
RTphaP2-R 5′-GTGAACTGGCTACGCAGGA-3′
RTphaP3-F 5′-ATTACGGCACCAAGGTCATC-3′
RTphaP3-R 5′-GTGGAGAGGTTCACGAGGTC-3′
RTphaP4-F 5′-GTGCGACTGATCCATTCTCC-3′
RTphaP4-R 5′-GTCCTTGAACTTGGCGTAGC-3′
RTphaR-F 5′-CTTCGAGCAGGAGAACAAGG-3′
RTphaR-R 5′-GATATTTCGGCACCACCATC-3′
RTsigA-F 5′-CAGGCGAAGGACAAGGAAAA-3′
RTsigA-R 5′-CGTCGGACAGATCGAGCAA-3′

Yoshida et al.

Yoshida et al. BMC Microbiology 2013 13:290   doi:10.1186/1471-2180-13-290

Open Data