Transcription profile of the genes deduced to be involved in PHB metabolism and accumulation. (A) Expression of the genes for PHB biosynthesis and degradation. qRT-PCR analysis was performed as described in the Methods, and data were normalized to constitutively expressed sigA as an internal control. Values are means of three independent results ± SD, and those followed by the same letters are not significantly different at the 95% confidence level. ND: no specific PCR product was detected as in the negative control experiment without reverse transcription, and thus was not taken into account for statistic analysis. (B) Expression of the four PhaP phasins. qRT-PCR analysis was performed and the results are presented as described for (A).
Yoshida et al. BMC Microbiology 2013 13:290 doi:10.1186/1471-2180-13-290