Figure 4.

Immunofluorescence analysis of TNF-α expression in gut. A: TNF-α expression was stimulated in larvae exposed to TNBS. TNF-α staining (red) and DAPI staining (blue) images were visualized by confocal laser scanning microscopy. Bars: 25 μm. B: TNF-α immunofluorescence levels increased with increasing concentrations of TNBS over time. All error bars represent as mean ± SEM, n=13–16 sections per group, aIndicates a significant difference (p<0.05) between TNBS-exposed group (25 μg/ml) and the control, bIndicates a significant difference (p<0.05) between TNBS-exposed group (50 μg/ml) and the control, cIndicates a significant difference (p<0.05) between TNBS-exposed group (75 μg/ml) and the control, dIndicates a significant difference (p<0.05) between control groups at 6 dpf and 4 dpf, eIndicates a significant difference (p<0.05) between control groups at 8 dpf and 4 dpf.

He et al. BMC Microbiology 2013 13:289   doi:10.1186/1471-2180-13-289
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