Effects of peptide length on recognition of VP4 peptides by antibodies raised against the first N-terminal 20 residues of EV71 VP4. The top panel shows the ELISA reaction of the polyclonal serum to peptides truncated at the carboxyl end of the 20-mer. The bottom shows the same with the truncations at the amino end, and the highlighted yellow region shows the minimal apparent “core” of the peptide for antibody recognition. The plus signs on the right of the diagram illustrate whether the polyclonal serum binds to the peptide fragment. OD450: optical density at 450 nm.
Zhao et al. BMC Microbiology 2013 13:287 doi:10.1186/1471-2180-13-287