Neonatal mice as a model to assess in-vitro neutralizing effects of anti-sera. Groups of one-day-old BALB/c suckling mice were inoculated intraperitoneally (i.p.) with the virus-sera and virus-PBS mixture. (A) Mice with different antiserum treatment at 11 days post-infection with EV71. The mouse on the left side received anti-chimeric VLPs sera and the one on the right side received anti-HBcAg VLPs sera. The appearance of limb paralysis in mouse is indicated by arrows. (B) Two representative mice in the PBS-treated group die at 7 days post-infection with EV71. (C) Survival rates were recorded daily after infection for 16 days. 10 mice were used for each group.
Zhao et al. BMC Microbiology 2013 13:287 doi:10.1186/1471-2180-13-287