Table 1

Infectious titre results obtained by RT-qPCR infectivity assay or plaque assay
A.
0-1 hr 1 day 3 days 6 days 7 days
RT-qPCR infectivity 7.50E + 06 7.28E + 06 4.35E + 06 3.35E + 06 2.43E + 06
Plaque assay 7.36E + 06 5.55E + 06 4.52E + 06 4.43E + 06 2.70E + 06
B.
RT-qPCR infectivity 3.06E + 06 1.14E + 06 2.14E + 06 1.30E + 06 3.78E + 05
Plaque assay 3.23E + 06 3.40E + 06 2.80E + 06 1.55E + 06 N/A

HSV529 test samples were incubated at A. 4–8°C or B. 22-25°C at various time points and the infectious titre was measured by RT-qPCR infectivity assay or plaque assay.

Azizi et al.

Azizi et al. BMC Microbiology 2013 13:284   doi:10.1186/1471-2180-13-284

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