Figure 1.

The accumulation of HSV529 RNA after post-infection. The infected cells were lysed after each time point (3, 6, 12, 16, and 24 hours post-infection), RNA was purified followed by DNase treatment, and RT-qPCR performed using specific primers for HSV-2. A. The accumulation of HSV529 targeting gD2 gene is shown. A linear relationship between the logarithm of the HSV529 concentration and the CT values were observed at 12 to 16 h post-infection. B. The accumulation of HSV529 targeting ICP-27 gene is shown No linear relationship between the logarithm of the HSV529 concentration and the CT values were observed at any time points (3, 6, 12, 16, and 24 h). As a representative, the accumulation of HSV529 RNA targeting ICP-27 after 6 h and 12 h post-infection is shown. C. The accumulation of HSV529 RNA targeting TK gene after 3 h and 6 h post-infection is shown. No linear relationship between the logarithm of the HSV529 concentration and the CT values were observed at any time points (3, 6, 12, 16, and 24 h). dil:dilution.

Azizi et al. BMC Microbiology 2013 13:284   doi:10.1186/1471-2180-13-284
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