Table 2

Strains and plasmids/cosmids used in this work
Strains/plasmids Description Reference
E. coli
DY380 ∆(mrr–hsdRMSmcrBC) mcrA recA1 λ cl857, ∆(cro–bioA)<>tet [46]
ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; carries RK2 derivative with defective oriT for plasmid mobilization, Kanr [45]
GM2929 dam-13::Tn9 dcm-6 hsdR2 recF143 M. Marinus, Univ. of Massachussetts Medical School
S. coelicolor A3(2)
M145 Prototrophic, SCP1- SCP2- Pgl+ [45]
J2401 M145 whiA::hyg [15]
J2408 M145 ∆whiH::ermE [15]
K300 M145 ∆SCO1774-1773::vph This work
K301 M145 ∆SCO1773::vph This work
K302 M145 ∆SCO3857::vph This work
K303 M145 ∆SCO4157::aac(3)IV This work
K316 M145 ∆SCO0934::aac(3)IV This work
K317 M145 ∆SCO7449-7451::aac(3)IV This work
K318 M145 ∆SCO1195-1196::Ωaac This work
K319 M145 ∆SCO4421::Ωaac This work
Plasmids/cosmids
pCR-BluntII Cloning vector Invitrogen
pIJ773 Source of apramycin resistance cassette, aac(3)IV, oriT [47]
pIJ780 Source of viomycin resistance cassette, vph, oriT [47]
pHP450Ωaac Source of apramycin resistance cassette, Ωaac [48]
pIJ2925 pUC-derived E. coli vector with a modified polylinker; bla [49]
pOJ260 Mobilizable vector, no replication or integration in S. coelicolor, Aprar [50]
pSET152 Mobilizable vector, integrates at ϕC31 attB site, Aprar [50]
pIJ82 Derivative of pSET152, Hygr Helen Kieser, JIC, Norwich, UK
pRT801 Mobilizable vector, integrates at ϕBT1 attB site, Aprar [51]
pIJ6902 Expression vector, thiostrepton-inducible tipAp promoter, integrates at ϕC31 attB site, Aprar [52]
pKF218 pRT801 containing SCO1775-1773 with part of upstream region This work
pKF219 pOJ260 containing SCO1775-1773 with part of upstream region This work
pKF278 pIJ82 containing SCO7449-7451 with part of upstream region This work
pKF210 Vector for cloning promoters upstream reporter gene encoding mCherry, based on pIJ6902 This work
pKF212 Promoter region of SCO0934 translationally fused to mCherry This work
pKF213 Promoter region of SCO1773 translationally fused to mCherry This work
pKF214 Promoter region of SCO1774 translationally fused to mCherry This work
pKF215 Promoter region of SCO3857 translationally fused to mCherry This work
pKF216 Promoter region of SCO4157 translationally fused to mCherry This work
pKF217 Promoter region of SCO4421 translationally fused to mCherry This work
M10 Cosmid containing SCO0934a [53]
I51 Cosmid containing SCO1773 and SCO1774a [53]
H69 Cosmid containing SCO3857a [53]
D84 Cosmid containing SCO4157a [53]
6 F11 Cosmid containing SCO4421a [53]
5C11 Cosmid containing SCO7449-7451a [53]
G11A Cosmid containing SCO1195-1196a [53]

aCosmid used to delete corresponding gene and to amplify promoter regions and regions used for complementation.

Salerno et al.

Salerno et al. BMC Microbiology 2013 13:281   doi:10.1186/1471-2180-13-281

Open Data