Table 1

Fluorescence-based assays of promoter activity
Average fluorescence intensity (arbitrary unit)
Spores Vegetative hyphae
Strain Avga 95CI Avga 95CIe
M145 19.0 16.2 - 21.9 3.51 -5.73 - 12.8
pKF210 21.3c 17.8 - 24.8 -11.1 -23.1 - 0.940
SCO0934b 68.7d 65.3 - 72.1 -18.7 -26.9 - -10.4
SCO1773b 35.5d 32.2 - 38.9 18.1 2.20 - 34.0
SCO1774b 1467d 1440 - 1493 14.3 1.39 - 27.2
SCO3857b 1077d 1048 - 1105 6.08 -2.98 - 15.1
SCO4157b 93.4d 90.1 - 96.7 12.33 4.39 - 20.3
SCO4421b 586d 568 - 604 6.02 2.04 - 10.0
SCO7449b 831d 805 - 856 15.7 8.87 - 22.5

aAverage intensity value per pixel after subtraction of background signals from the medium. The fluorescence intensity was measured in areas of 0.22 μm2 per spore (totally between 454–743 spores per strain) and in 50 randomly selected areas (0.22 μm2) of the surrounding medium.

bPromoter region of corresponding gene translationally fused to the gene encoding the fluorescent protein mCherry (mCh) in pKF210, integrated into the chromosome of M145.

cDifference from M145 not significant (P = 0.37) according to Student’s t test.

dDifference from M145/pKF210 highly significant (P > 0.001) according to Student’s t test.

e95% confidence interval.

Salerno et al.

Salerno et al. BMC Microbiology 2013 13:281   doi:10.1186/1471-2180-13-281

Open Data