Table 3

Detection of the atpE gene (locus Rv1305 in M. tuberculosis genome) in different Mycobacterium species (25 ± 15 ng of DNA) and non-mycobacterial microorganisms (50 ± 15 ng of DNA)
Microorganism codificationa Microorganism Results
A CPS MC13 M. arupense Detected
CPS MC11 M. austroafricanum Detected
ATCC 25291T M. avium subsp. avium Detected
CIP 1173/P2 M. bovis (BCG) Detected
ATCC 19977T M. chelonae spp. abscessus Detected
ATCC 35752T M. chelonae spp. chelonae Detected
CIP 105388 T M. gadium Detected
ATCC 14470T M. gordonae Detected
ATCC 6841T M. fortuitum spp. fortuitum Detected
CPS MC8 M. insubricum Detected
ATCC 15985T M. intracellulare Detected
ATCC 12478T M. kansasii Detected
CIP 105465T M. lentiflavum Detected
THAI 53 M. leprae Detected
CPS MC10 M. llatzerense Detected
ATCC 927T M. marinum Detected
CIP 105223T M. mucogenicum Detected
CIP 106811T M. nonchromogenicum Detected
CPS MC6 M. psychrotolerans Detected
ATCC 14467T M. peregrinum Detected
CPS MC9 M. porcinum Detected
CIP 105416T M. scrofulaceum Detected
CPS MC7 M. setense Detected
ATCC 25275T M. simiae Detected
ATCC 19420T M. smegmatis Detected
ATCC 35799T M. szulgai Detected
CIP 104321T M. terrae Detected
CIP 106368 M. tusciae Detected
ATCC 25618T M. tuberculosis (H37Rv) Detected
CPS CR08085632 M. ulcerans Detected
ATCC 19250T M. xenopi Detected
B CMR SC10 Acinetobacter sp. ND
CMR SC9 Aeromonas sp. ND
CMR SC23 Arthrobacter sp. ND
CMR SC44 Aspergillus sp. ND
CMR SC5 Bacillus sp. ND
CMR SC24 Brevundimonas sp. ND
ATCC 6871T C. ammoniagenes ND
ATCC 13032T C. glutamicum ND
ATCC 10700T C. pseudodiphtheriticum ND
CMR SC35 Escherishia sp. ND
CMR SC19 Flavobacterium sp. ND
ATCC 43504T Helicobacter pylori ND
CMR SC45 Kocuria sp. ND
CMR SC31 Leuclercia sp. ND
CMR SC28 Leucobacter sp. ND
CMR SC29 Microbacterium sp. ND
CMR SC3 Micrococcus sp. ND
DSM 44546T N. cerradoensis ND
DSM 44490T N. cummidelens ND
IFM 10152 N. farcinica ND
CMR SC42 Penicillium sp. ND
CMR SC1 Pseudomonas sp. ND
CMR SC26 Rhodococcus sp. ND
CMR SC34 Serracia fonticola ND
CMR SC22 Solibacillus sp. ND
CMR SC12 Staphylococcus caprae ND
CMR SC6 Staphylococcus hominis ND
CMR SC46 Staphylococcus lugdunensis ND
CMR SC49 Streptomyces sp. ND
CMR SC41 Trichoderma sp. ND

TaqMan® real-time PCR amplification was performed using forward primer FatpE, reverse primer RatpE and probe PatpE in duplicate assays. ND stands for not detected sigmoidal curve.

aATCC: American Type Culture Collection; CPS: Collection de la Pitié-Salpêtrière, Paris, France; T: type strain; CIP: Collection de l′Institut Pasteur, Paris, France; CMR: Collection de Microorganismes de Radomski et al. 2010 [17], Paris, France; DSM: DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany; IFM: Culture Collection of the Research Centre for Pathogenic Fungi and Microbial Toxicoses, Chiba, Japan.

Radomski et al.

Radomski et al. BMC Microbiology 2013 13:277   doi:10.1186/1471-2180-13-277

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