Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Description Reference
V. anguillarum strains
M93sm Spontaneous Smr mutant of M93 (serotype O2a); parental strain isolated from a diseased ayu (Plecoglossus altivelis) from Lake Biwa, Japan [2]
JR1 Smr Cmrvah1; insertional vah1 mutant of M93Sm [8]
XM21 Smr Cmr Tcrvah1+; vah1 complement strain of JR1 This study
S262 Smr Cmrplp; insertional plp mutant of M93Sm This study
XM31 Smr Cmr Tcrplp+; plp complement strain of S262 This study
S123 Smr CmrrtxA; insertional rtxA mutant of M93Sm [9]
JR3 Smr Cmr Kmrvah1 plp; insertional vah1mutant of JL01 [8]
S183 Smr Cmr Kmrvah1 rtxA; insertional rtxA mutant of S171 [9]
XM62 Smr Cmr Kmr Tcrvah1+ rtxA; vah1 complement strain of S183 This study
S187 Smr Cmr Kmrplp rtxA; insertional rtxA mutant of JL01 This study
XM90 Smr Cmr Kmrvah1 plp rtxA; insertional plp mutant of S264 This study
XM93 Smr Cmr Kmr Tcrvah1 plp + rtxA; plp complement strain of XM90 This study
JL01 Smr Kmrplp; mini-Tn10Km insertion into plp [8]
S171 Smr Kmrvah1; allelic exchange vah1 mutant [9]
S264 Smr Kmrvah1 rtxA; allelic exchange vah1 and rtxA mutant This study
E. coli strains
Sm10 thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λ pir) [21]
S253 Kmr Cmr; Sm10 containing plasmid pNQ705-plp This study
S118 Kmr Cmr; Sm10 containing plasmid pNQ705-rtxA [9]
S250 Kmr Cmr; Sm10 containing plasmid pDM4-rtxA5' This study
S252 Kmr Cmr; Sm10 containing plasmid pDM4-rtxA5'-rtxA3' This study
U21 Kmr Apr Tcr; Sm10 containing plasmid pSUP202-vah1 This study
U31 Kmr Apr Tcr; Sm10 containing plasmid pSUP202-plp This study
M15 NalS StrS RifSthilacara+gal+mtl FrecA+uvr+lon+ (pREP4) QIAGEN, USA
S238 Kmr Apr; M15 containing plasmid pQE30UA-plp This study
S269 Kmr Apr; M15 containing plasmid pQE60-plp This study
Plasmid
PCR2.1 Kmr Apr; Cloning vector Invitrogen, USA
pNQ705-1 Cmr; suicide vector with R6K origin [22]
pNQ705-vah1 Cmr; for insertional vah1mutation [8]
pNQ705-plp Cmr; for insertional plp mutation This study
pNQ705-rtxA Cmr; for insertional rtxA mutation [9]
pDM4 Cmr SacBCr; suicide vector with R6K origin [11]
pDM4-rtxA5'-rtxA3' Cmr SacBCr; for allelic exchange rtxA mutation This study
pSUP202 Cmr Apr Tcr; E. coliV. anguillarum shuttle vector [21]
pSUP202-vah1 Apr Tcr; for complementation of vah1 This study
pSUP202-plp Apr Tcr; for complementation of plp This study
pQE-30 UA Apr; expression vector with N-terminal His6-tag QIAGEN, USA
pQE30UA-plp Apr; for expression of rPlp that is used to make anti-Plp This study
pQE60 Apr; expression vector with C-terminal His6-tag QIAGEN, USA
pQE-60-plp Apr; for expression of rPlp for enzymatic activity analysis This study

Li et al.

Li et al. BMC Microbiology 2013 13:271   doi:10.1186/1471-2180-13-271

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