rPlp activity determined by TLC analysis. BPC (A), NBD-PE (B), NBD-PS (C), and NBD-Sm (D) were used as phospholipid substrates to examine the specificity of rPlp. Phosphate-buffered saline (PBS) was used as a negative control, and PLA2 enzyme from porcine pancreas as a positive control. BPC: BODIPY-labeled phosphatidylcholine; BLPC: BODIPY-labeled lysophosphatidylcholine; NBD-PE: NBD-labeled phosphatidylethanolamine; NBD-LPE: NBD-labeled lysophosphatidylethanolamine; NBD-PS: NBD-labeled phosphatidylserine; NBD-FFA: NBD-labeled free fatty acid; NBD-SM: NBD-labeled sphingomyelin; NBD-CE: NBD-labeled ceramide.
Li et al. BMC Microbiology 2013 13:271 doi:10.1186/1471-2180-13-271