Open Access Highly Accessed Methodology article

Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes

Devin W Close1, Fortunato Ferrara2, Armand EK Dichosa1, Sandeep Kumar1, Ashlynn R Daughton1, Hajnalka E Daligault1, Krista G Reitenga1, Nileena Velappan1, Timothy C Sanchez1, Srinivas Iyer1, Csaba Kiss1, Cliff S Han1 and Andrew RM Bradbury1*

  • * Corresponding author: Andrew RM Bradbury amb@lanl.gov

  • † Equal contributors

Author Affiliations

1 Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA

2 New Mexico Consortium, Los Alamos, NM, USA

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BMC Microbiology 2013, 13:270  doi:10.1186/1471-2180-13-270

Published: 27 November 2013

Additional files

Additional file 1:

Sequence alignment of the four scFvs selected against L. acidophilus. HCDR3 sequences are highlighted in yellow.

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Additional file 2:

Binding of the four unique anti-La scFvs to different Lactobacillus species using scFv culture supernatant and flow cytometry. The anti-La scFvs are all specific to L. acidophilus and the anti-La2 may discriminate between L. acidophilus strains.

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Additional file 3:

Bacteria identified in various gates after single cell sorting and classification. Approximately 88 cells were sorted from each gate for each replicate. Species identities reported at >94% maximum identity by Blastn search of the 16S rDNA sequences. Replicates are different bacteria preps isolated from yogurt cultures and the gates correspond to gates shown in Figure 4 of the main text.

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