Table 1

Strains and plasmids used in this study
Strains or plasmids Description Source (Reference)
Pseudomonas aeruginosa
PAO1 Prototrophic strain [31]
PW5661 PA2783-E12::ISlacZ/hah; insertion at bp 27 of 1800 in the ORF; Tcr [28]
PAOΔvfr (PAO9001) vfr deletion of PAO1; Gmr [32]
PAO-R1 ΔlasR::tet derivative of PAO1; Tcr [33]
Escherichia coli
DH5α Φ80lacZΔM15 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 Invitrogen
CC118 F Δ(ara-leu)7697 araD139 ΔlacX74 galE galK ΔphoA20 thi rpsE rpoB argE(Am) recA1 appR1 [34]
CC102 F42 lacI3 zzf-2::TnphoA/CC118 [34]
LMG194 F ΔlacX74 galE galK thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10 Invitrogen
Plasmids
pUCP19 E. coli-P. aeruginosa shuttle vector; Cbr [35]
pKF917 pUCP19 carrying vfr; Cbr [15]
pCR™2.1-TOPO® 3.9 kbp TA cloning vector; Cbr, Kmr Invitrogen
pAB1 pCR2.1-TOPO carrying PA2783; Cbr , Kmr This study
pAB2 pUCP19 carrying PA2783 expressed from Plac; Cbr This study
pAB3 pAB2 carrying a phoA fusion; Cbr, Kmr This study
pBAD/HisC pBR322-derived expression vector in which cloned genes are expressed from the araBAD promoter (PBAD); Cbr Invitrogen
pAB4 pBAD/HisC carrying PA2783 expressed from PBAD; Cbr This study

ORF, open-reading frame; r, resistant; Cb, carbenicillin; Gm, gentamicin; Km, kanamycin; Tc, tetracycline.

Balyimez et al.

Balyimez et al. BMC Microbiology 2013 13:269   doi:10.1186/1471-2180-13-269

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