PA2783 is exported to the outer membrane in PAO1. Overnight cultures of PAO1 were subcultured in LB broth and grown to the time points indicated on the graph. Cells from 1-ml samples were fractionated and the level of alkaline phosphatase activity in the outer membrane fraction was determined as previously described. Values represent the means of three independent experiments ± SEM. ***P <0.001.
Balyimez et al. BMC Microbiology 2013 13:269 doi:10.1186/1471-2180-13-269