Figure 4.

PA2783 is exported to the outer membrane in PAO1. Overnight cultures of PAO1 were subcultured in LB broth and grown to the time points indicated on the graph. Cells from 1-ml samples were fractionated and the level of alkaline phosphatase activity in the outer membrane fraction was determined as previously described. Values represent the means of three independent experiments ± SEM. ***P <0.001.

Balyimez et al. BMC Microbiology 2013 13:269   doi:10.1186/1471-2180-13-269
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