Vfr regulates the transcription of PA2782 and PA2783 at early and late stages of growth of PAO1. PAO1 and PAOΔvfr strains were grown in LB broth overnight and subcultured into fresh LB broth to a starting OD600 of 0.02. Cells were harvested at 4 h and 6 h, OD600 of 0.37 and 0.79 for PAO1 and 0.41 and 0.89 for PAOΔvfr, respectively, and total RNA was extracted. Levels of PA2782 and PA2783 mRNA in each sample were determined by qRT-PCR using specifically-designed primers. Values represent the means of three independent experiments ± SEM. ***P <0.001.
Balyimez et al. BMC Microbiology 2013 13:269 doi:10.1186/1471-2180-13-269