Open Access Highly Accessed Research article

Characterization of the Pseudomonas aeruginosa metalloendopeptidase, Mep72, a member of the Vfr regulon

Aysegul Balyimez1, Jane A Colmer-Hamood2, Michael San Francisco1 and Abdul N Hamood2*

Author Affiliations

1 Biology Department, Texas Tech University, Lubbock, TX, USA

2 Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA

For all author emails, please log on.

BMC Microbiology 2013, 13:269  doi:10.1186/1471-2180-13-269

Published: 27 November 2013

Additional files

Additional file 1:

Oligonucleotides used in this study.

Format: PDF Size: 89KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Amino acid homology of the predicted PA2783 protein endopeptidase domain with other bacterial proteins.

Format: PDF Size: 18KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

The predicted PA2783 protein carries two carbohydrate-binding modules. Interrogation of the non-redundant databases at NCBI (http://www.ncbi.nlm.nih.gov/ webcite; accessed 06/19/2013) using BLASTP revealed homology with the CBM_4_9 family (Cdd:pfam02018) of diverse CHO-binding proteins. CHO-binding domain I (A) and domain II (B), have different aa sequences but both were strongly homologous to the two CHO-binding modules of the Pseudomonas mendocina (Pmendo) carbohydrate-binding CenC domain-containing protein and the Ni,Fe-hydrogenase I small subunit of Hahella chejuensis (Hcheju). For the pfam, identical aa are indicated by * and similar aa by ^; for bacterial proteins, identical aa are shown in red, similar aa in blue, and non-similar aa in black; Pmucil, Paenibacillus mucilaginosus; Clen-1 and Clen-2, Cellulosilyticum lentocellum CHO-binding domains I and II. Percentages of aa identity and similarity are shown in Additional file 4.

Format: PDF Size: 392KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Amino acid homology of the predicted PA2783 protein carbohydrate-binding domains I and II with other bacterial proteins.

Format: PDF Size: 16KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data