Altered membrane lipid composition of strain PDJ28 following the removal of the glycerol supplement. Strain PDJ28 (ΔgpsA) was labeled with [14C]acetate in the presence of glycerol to an OD600 of 0.6. The cells were then washed and resuspended in media either with (A) or without (B) the glycerol supplement, and after 180 min at 37°C, the cellular lipid composition was determined by 2-dimensional thin-layer chromatography of the extracted lipids. The distribution of radioactivity was determined using a PhosphoImager screen and a Typhoon 9200.
Parsons et al. BMC Microbiology 2013 13:260 doi:10.1186/1471-2180-13-260