Quantitative measurement of the reduced biofilm formation with KSL-W.C. albicans was cultured on a 3D porous scaffold in the presence of KSL-W for 2, 4, and 6 days. After each culture period, the samples were supplemented with XTT solution and incubated for 5 h at 37°C. The absorbance at 450 nm was measured to quantify XTT metabolic product intensity proportional to the number of viable cells. (A) 2 days; (B) 4 days; (C) 6 days. Results are means ± SD for three different separate experiments.
Theberge et al. BMC Microbiology 2013 13:246 doi:10.1186/1471-2180-13-246