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Open Access Research article

Comparison of the effects of hyaluronidase and hyaluronic acid on probiotics growth

Alessandro Di Cerbo1*, Maria Aponte2, Rita Esposito1, Moreno Bondi3 and Beniamino Palmieri1

Author Affiliations

1 Dipartimento di Chirurgia Generale e Specialità Chirurgiche, Università degli Studi di Modena e Reggio Emilia, via del pozzo 71 41124, Modena, Italy

2 Istituto di Scienze dell’Alimentazione, ISA-CNR, Via Roma, 64, 83100, Avellino, Italy

3 Dipartimento di Scienze della vita, Università degli Studi di Modena e Reggio Emilia, Via Campi 287, 41125, Modena, Italy

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BMC Microbiology 2013, 13:243  doi:10.1186/1471-2180-13-243

Published: 4 November 2013



Hyaluronic acid has several clinical applications. Recent evidences suggested antimicrobial properties against several pathogens. The aim of the present survey was to evaluate the effect of hyaluronic acid, alone or in combination with hyaluronidase, on protechnological or probiotic strains.


The role of hyaluronic acid and hyaluronidase on in vitro growth rate of different lactic acid bacteria was investigated. Standard methods revealed that low concentrations of hyaluronic acid (0.5-0.125 mg ml-1), and hyaluronidase at fixed concentration (1.6 mg ml-1), resulted in an increased bacterial strains growth up to 72 hours whereas higher concentrations of the acid (2 and 1 mg ml-1), and hyaluronidase at the same fixed concentration, reduced the bacterial growth.


Observations might suggest a possible protective role of both hyaluronidase and low doses of hyaluronic acid towards some strains, supporting their in vivo proliferation and engraftment after oral administration. Hyaluronidase introduction into growth medium greatly enhanced the bacterial growth up to 72 hours.

Hyaluronic acid; Hyaluronidase; Lactic acid bacteria