MazF6-HSV toxin does not physically interact with non-cognate MazE-HIS antitoxinsin vivo. With all pairs tested, MazE-HIS antitoxins are present in the elution fractions, while MazF6-HSV is present in the unbound flow and wash fractions only, indicating that MazF6-HSV is only able to physically interact with its cognate antitoxin, MazE6. From top to bottom, antitoxins tested are: MazE1 (Rv0456b), MazE2 (Rv0660c), MazE3 (Rv1103c), MazE4 (Rv1494), MazE5 (Rv1943c), MazE7 (Rv2063), MazE9 (Rv2801a).
Ramirez et al. BMC Microbiology 2013 13:240 doi:10.1186/1471-2180-13-240