Validation of antibody response (serum IgG and intestinal sIgA). Serum and gut wash from mice treated with PBS and vaccinated with MT4 and MT5 were collected, diluted to a highest dilution of 1:120 (serum) and 1:9 (gut wash). The presence of Salmonella specific IgG and secretory IgA were detected by Western blots. The representative Western blot analysis of the antibody responses was done by developing the blots of overnight grown cultures of MT5, MT4, SB300 (wild-type S. Typhimurium) and M1525 (S. Enteritidis; negative control) with the serum and gut wash of the immunized mice.
Pati et al. BMC Microbiology 2013 13:236 doi:10.1186/1471-2180-13-236