Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Phenotypes and/or characteristics Reference or source
B. cenocepacia
WT J2315 Wild type strain, Genomovars III of B. cepacia complex ATCC
d0581 BDSF-minus mutant derived from J2315 with Bcam0581 being deleted 35
d0581(0581) Mutant d0581 harboring the expression construct pMLS7-0581 35
P. aeruginosa
PA14 Clinical isolate Laboratory collection
PA14(PCZ) PA14 harboring the T3SS reporter construct mini-CTX-lacZ with the exsCEBA promoter fused to lacZ This study
PA14 (PlasR-lacZ) PA14 harboring the reporter construct pME2PlasR-lacZ This study
PA14 (PpqsR-lacZ) PA14 harboring the reporter construct pME2PpqsR-lacZ This study
PA14 (PrhlR-lacZ) PA14 harboring the reporter construct pME2PrhlR-lacZ This study
dpsrA (PCZ) psrA deletion mutant harboring the T3SS reporter construct mini-CTX-lacZ with the exsCEBA promoter fused to lacZ This study
dPA1396 PA1396 deletion mutant derived from PA14 This study
E.coli
S17-1 Res- pro mod+ integrated copy of RP4, mob+, used for incorporating Laboratory collection
CV026 The biosensor strain of C4-HSL Laboratory collection
Agrobacterium tumefaciens
CF11 AHL reporter strain Lab of Stephen K. Farrand
Plasmid
PCZ mini-CTX-lacZ with the exsCEBA promoter fused to lacZ 62
PlasR-lacZ pME2-lacZ containing promoter of lasR This study
PpqsR-lacZ pME2-lacZ containing promoter of pqsR This study
PrhlR-lacZ pME2-lacZ containing promoter of rhlR This study

Deng et al.

Deng et al. BMC Microbiology 2013 13:231   doi:10.1186/1471-2180-13-231

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