Agarose gel electrophoresis and Southern blot hybridization of DNA preparations of 18 STEC strains. A) plasmid preparations (left side) and Southern blot hybridization with a subAB1 specific DNA probe (right side). Gene Ruler 1 kb DNA ladder (M), Lambda-Mix Marker 19 (Mλ) (both Fermentas), K17 (lane 1), LM25602/08 (2), CB11588 (3), CB11633 (4), TS20/08 (5), TS26/08 (6), SF16b (7) TS18/08 (8), TS30/08 (9), EDL933 (10). B) chromosomal DNA (left side) and Southern blot hybridization with a subAB2 specific DNA probe (right side). Gene Ruler 1 kb DNA ladder (M), Lambda DNA/HindIII Marker (MλH) (Fermentas), LM14603/08 (1), LM16092/08 (2), LM227553stx1 (3), LM227553stx2 (4), LM27564 (5), LM27558 (6), LM27555 (7), LM14960 (8), LM27558 (9). EDL933 (10) was used as a negative control for hybridization. Recombinant plasmid pK18 containing subAB1 was used as positive control for hybridization (data not shown).
Funk et al. BMC Microbiology 2013 13:230 doi:10.1186/1471-2180-13-230