Relative fold change for genes PAL1, MFS, ERG3, GLN1, FKS1, KRE6, CAP20 and GAC1. (A) Gene expression profile of Paracoccidioides yeast cells exposed to OenB after 90 and 180 min. (B) GAC1 gene expression of Paracoccidioides yeast cells exposed to a causative agent osmotic stress, KCl. (C) Gene expression profile of Paracoccidioides yeast cells used to infect macrophage cells exposed to OenB after 24 h. Changes in gene expression levels were calculated by the relative standard curve method using the non-treated control samples as the calibrator. Each error bar represents the standard error of the mean (±SD), and significant-fold changes are denoted by asterisks in the figure (*p ≤ 0.05). Data were normalized with the transcript encoding the α-tubulin protein.
Zambuzzi-Carvalho et al. BMC Microbiology 2013 13:227 doi:10.1186/1471-2180-13-227