Table 2

Comparison of experimentally determined eliminations from N-terminal AspN digested/tryptic peptides of LprF, LpqH, LpqL and LppX in the MALDI-TOF/TOF spectra of BCG parental and Δlnt mutant strain with theoretically calculated eliminations
Modification Eliminated fragment Calculated mass of eliminated fragment [ Da ] Experimentally determined mass of eliminated fragment [ Da ]
Parental strain Δlnt
LprF LpqH LpqL LppX LprF LpqH LpqL LppX
C16/C19 C16 C16/C19 C19 C16/C19 C16 C16/C19 C16 C16/C16 C16/C19 C16/C16 C16/C18 C16/C19 C16/C19
O-linked palmitoyl (C16) Palmitic acid 256.24 256.5 - 256.3 256.3 n.d. * - - 256.2 256.1 256.3 256.3 n.d. *
O-linked oleyl (C18) Oleic acid 282.24 - - - - n.d. * - - - 282.4 - - n.d. *
O-linked tuberculostearyl (C19) Tuberculostearic acid 298.29 - - 298.3 298.3 n.d. * - - - - 298.3 298.4 n.d. *
N-linked palmitoyl (C16) + Didehydroalanine Palmitamide + Didehydroalanine 307.26 - 306.6 - - n.d. * - - - - - - n.d. *
N-linked tuberculostearyl (C19) + Didehydroalanine Tuberculostearinamide + Didehydroalanine 349.31 349.8 - - - n.d. * - - - - - - n.d. *
Diacylglyceryl (C16/C16) Diacylhioglyceryl (C16/C16) 584.44 - - - - n.d. * 583.3 - - - - - n.d. *
Diacylglyceryl (C16/C18) Diacylhioglyceryl (C16/C18) 610.52 - - - - n.d. * - - - - - - n.d. *
Diacylglyceryl (C16/C19) Diacylhioglyceryl (C16/C19) 626.53 625.9 626.7 626.7 626.6 n.d. * - 626.7 - - 626.6 626.7 n.d. *
C16 fatty acid α-thioglyceryl ester 328.24 - - 328.4 328.3 n.d. * - - - - - n.d. *
C19 fatty acid α-thioglyceryl ester 370.29 - - 370.5 370.3 n.d. * - 369.8 - - - 370.4 n.d. *
Hexose Hexose 160.76 161.62 - - - n.d. * - 162.9 - - - - n.d. *

* MALDI-TOF/TOF data for LppX from M. bovis BCG were not determined, since MS data of LppX from this study are comparable with data of LppX from M. smegmatis (A. Tschumi et al. 2009).

Brülle et al.

Brülle et al. BMC Microbiology 2013 13:223   doi:10.1186/1471-2180-13-223

Open Data