Secretion requires an intact signal sequence. Five secreted proteins (CBU0110, CBU0915, CBU1135, CBU1173 and CBU1984) without their respective signal sequence were expressed as described in Figure 2. Pellets and TCA precipitated supernatants were analyzed by immunoblotting using antibody directed against the FLAG-tag.
Stead et al. BMC Microbiology 2013 13:222 doi:10.1186/1471-2180-13-222