Table 2

List of beer proteins identified by MALDI-TOF-MS and MS/MS
Theoretical values
Spot ID Protein name Accession no. Mr(Da) pI Scorea Sequence coverage (%) No. of peptide MS/MS (sequnece of matched peptides)b
A6 Protein Z-type serpin gi|1310677 43307 5.61 110 32 11
A8 Protein Z-type serpin gi|1310677 43307 5.61 156 22 12
A9 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 107 29 7
A10 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 127 38 10
A12 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 86 58 9
A16 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 100 53 8
A17 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 98 53 8
A18 D-hordein gi|671537 51154 7.60 207 9 6
A19 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 91 33 8
A22 Lipid transfer protein 1 gi|19039 10145 8.91 243 21 4
A24 Lipid transfer protein 1 gi|19039 10145 8.91 296 68 5
A25 Lipid transfer protein 1 gi|19039 10145 8.91 100 68 6
A26 Lipid transfer protein 1 gi|19039 10145 8.91 128 93 7
A28 Lipid transfer protein 2 gi|128377 10806 6.78 77 37 4
A29 Lipid transfer protein 2 gi|128377 10806 6.78 72 37 4
B1 Uth1 gi|486485 47576 4.45 90 4 1 K.TQWPSEQPSDGR.S
B2 Exg1 gi|37926403 47335 4.45 257 23 9
B3 Protein Z-type serpin gi|1310677 43307 5.61 178 27 9
B4 Protein Z-type serpin gi|1310677 43307 5.61 118 33 11
B6 Protein Z-type serpin gi|1310677 43307 5.61 178 27 9
B8 Protein Z-type serpin gi|1310677 43307 5.61 120 26 10
B9 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 110 54 8
B10 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 98 52 7
B12 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 109 55 9
B16 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 115 29 5
B17 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 94 53 8
B19 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 99 15 3
B21 Lipid transfer protein 1 gi|19039 10145 8.91 252 52 6
B23 Lipid transfer protein 1 gi|19039 10145 8.91 595 74 8
B24 Lipid transfer protein 1 gi|19039 10145 8.91 103 52 6
B25 Lipid transfer protein 1 gi|19039 10145 8.91 493 52 6
B26 Lipid transfer protein 1 gi|19039 10145 8.91 366 57 6
C2 Exg1 gi|37926403 47335 4.45 254 20 7
C3 Protein Z-type serpin gi|1310677 43307 5.61 223 25 9
C4 Protein Z-type serpin gi|1310677 43307 5.61 278 20 8
C5 Bgl2 gi|6321721 34118 4.16 154 6 1 R.NDLTASQLSDKINDVR.S
C6 Protein Z-type serpin gi|1310677 43307 5.61 118 21 8
C7 Protein Z-type serpin gi|1310677 43307 5.61 154 25 11
C8 Protein Z-type serpin gi|1310677 43307 5.61 120 23 10
C9 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 167 55 9
C10 Trypsin/amylase inhibitor pUP13 gi|225102 15370 5.35 104 50 7
C14 Trypsin inhibitor Cme precursor gi|1405736 16341 7.49 99 29 5
C15 Trypsin inhibitor Cme precursor gi|1405736 16341 7.49 144 29 5
C16 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 211 38 7
C17 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 220 25 6
C19 Alpha-amylase inhibitor BDAI-I gi|123970 14045 5.36 182 25 5
C22 Lipid transfer protein 1 gi|19039 10145 8.91 141 75 5
C23 Lipid transfer protein 1 gi|19039 10145 8.91 223 40 3
C24 Lipid transfer protein 1 gi|19039 10145 8.91 220 58 4
C25 Lipid transfer protein 1 gi|19039 10145 8.91 241 68 4
C26 Lipid transfer protein 1 gi|19039 10145 8.91 178 50 4

aProtein identifications were confirmed with a significant MASCOT score of 71 for peptide mass fingerprint and ANOVA p ≤ 0.05, and a minimum of three matched peptides.

bSignificant MS/MS score is > 54 for searches in Saccharomyces cerevisiae. Spectra’s for single peptide identifications are supplied in Additional file 1.

Berner et al.

Berner et al. BMC Microbiology 2013 13:215   doi:10.1186/1471-2180-13-215

Open Data