Standard curves for the intergenic gyrA/gyrB region (a) and the ITS- (b) and intergenic region (c) in AcH 505 and P. croceum respectively. Serial dilutions of plasmids with the target DNA insert were used in individual qRT-PCR assays to generate the standard curves. The R2 values, slopes and efficiencies are shown for each reaction.
Kurth et al. BMC Microbiology 2013 13:205 doi:10.1186/1471-2180-13-205