Table 1

Up- or down-regulated Brucella suis proteins under nutrient starvation conditions
Spot IDa ORFb Protein functionc Theoret.Mr/pId Fold changee t-Testf
Adaptation to atypical conditions
2146 BR2149 Dps family protein (DNA-binding proteins from starved cells) 18.2/5.3 2.63 0.00019
429 BR0685 organic solvent tolerance, putative 88.7/5.4 1.53 0.024
2122 BR2149 Dps family protein 18.2/5.3 1.52 0.006
438 BR0685 organic solvent tolerance, putative 88.7/5.3 1.49 0.0004
Stress proteins/chaperones, protein folding
1624 BR0171 heat shock protein GrpE 25.2/4.7 −1.42 0.039
662 BR2125 chaperone protein DnaK 68.2/4.9 1.65 0.0056
Cell envelope
1653 BRA0423 31 kDa outer-membrane immunogenic protein (“Omp31-2”) 23.2/5.2 1.45 0.00034
1874 BRA0423 31 kDa outer-membrane immunogenic protein (“Omp31-2”) 23.2/5.2 1.34 0.026
Transport and binding proteins
1415 BR0639 porin Omp2a (omp2b) 40.5/4.6 1.41 0.03
1410 BR0639 porin Omp2a (omp2b) 40.5/4.6 1.4 0.028
2176 BRA0565 bacterioferritin 18.7/4.6 1.38 0.00065
1229 BRA0655 glycerol-3-phosphate ABC transporter, periplasmic 47.2/5.4 1.33 0.0043
Energy metabolism
ATP-proton motive force interconversion
1019 BR1800 ATP synthase F1, gamma subunit 32.0/7.8 1.6 0.021
Electron transport
1435 BRA0893 thioredoxin 34.7/4.8 −1.34 0.0045
Glycolysis/TCA cycle
1145 BR1132 enolase 45.4/5.0 1.43 0.0021
Amino acid metabolism
Biosynthesis
1915 BRA0883 3-isopropylmalate dehydratase, small subunit 22.5/5.0 −1.55 0.0013
221 BR1488 carbamoyl-phosphate synthase, large subunit 126.9/5.0 −1.34 0.0098
Degradation
278 BRA0725 glycine cleavage system P protein 99.9/5.8 1.51 0.00044
Transport
1219 BRA1193 amino acid ABC transporter 44.2/5.6 1.38 0,000015
1293 BRA0953 amino acid ABC transporter, periplasmic amino acid-binding protein, putative 43.3/5.3 1.36 0.0019
1549 BR0741 amino acid ABC transporter, periplasmic amino acid binding protein 37.2/5.3 1.31 0.00014
Protein metabolism
Biosynthesis
1783 BR0455 ribosomal protein S6 17.1/8.0 1.69 0.0069
1980 BR0452 ribosomal protein L9 21.0/4.8 1.59 0.00041
Secretion
313 BR1945 preprotein translocase, SecA subunit 103.0/5.1 −1.34 0.005
DNA/RNA metabolism
Biosynthesis
221 BR1488 carbamoyl-phosphate synthase, large subunit 126.9/5.0 −1.34 0.0098
454 BR0837 phosphoribosylformylglycinamidine synthase II 80.0/4.8 −1.31 0.01
456 BR0837 phosphoribosylformylglycinamidine synthase II 80.0/4.8 −1.31 0.015
Degradation
689 BR2169 polyribonucleotide nucleotidyltransferase 77.7/5.0 1.55 0.0029
Fatty acid metabolism
Degradation
1881 BR1510 long-chain acyl-CoA thioester hydrolase, putative 14.25/6.6 1.67 *
Sugar metabolism
Transport
1642 BR0544 ribose ABC transporter, periplasmic D-ribose-binding 34.6/4.8 1.46 *
Regulation
1743 BR0569 transcriptional regulator, Ros/MucR family 16.10/7.8 1.73 0.021
1843 BR2159 transcriptional regulator, Cro/Cl family 15.1/9.0 1.6 *
1813 BR1502 leucine-responsive regulatory protein 17.8/6.7 1.5 0.049
Oxidoreduction
1975 BRA0708 alkyl hydroperoxide reductase C 20.6/5.0 −1.39 0.005
Cofactor biosynthesis
826 BRA0491 8-amino-7-oxononanoate synthase 40.6/7.3 1.52 0.033
Unknown function
2190 BRA0336 conserved hypothetical protein 18.4/5.0 −1.42 0. 022

aThe indicated number is an arbitrary designation of the annotated spots on the 2D proteome maps [see Additional files 1 and 2].

bOpen reading frame number attributed by Paulsen et al. [20].

cAs annotated by Paulsen et al. [20].

dCalculated from the amino acid sequence of the translated open reading frame.

eIncrease or decrease of protein concentrations after normalization of protein spot intensities from 2D-DIGE gels of B. suis recovered from a 6-weeks-starvation condition as compared to normalized protein spot intensities of corresponding spots from early stationary phase control of B. suis in TS broth.

fStatistical significance of the ratio described ine.

*indicates lack of statistical validation of the ratio described ine, but increase in protein concentration was observed in each of the three independent experiments.

Al Dahouk et al.

Al Dahouk et al. BMC Microbiology 2013 13:199   doi:10.1186/1471-2180-13-199

Open Data