Table 3

Amino acid similarity of the L. pneumophila Sg1 specific LPS-biosynthesis region from lpg0769-lpg0761 (ORF 1 - ORF 15) of strain Philadelphia 1 to other Sg1 strains
Amino acid similarity [%]*
Gene name of L. pneumophila Philadelphia# Knoxville# Benidorm# Bellingham# Allentown# OLDA# Camperdown# Heysham#
Sg1 strain Philadelphia 1 Paris 2300/99 Alcoy Corby Uppsala 3 Ulm 130b Lens HL 0604 1035 Görlitz 6543 Lorraine RC1 Camperdown 1 Heysham1
lpg0761 (galE) ORF 15 100 100 100 100 100 97.1 96.0 99.8 99.8 99.8 100 100 100
lpg0762 (wecA) ORF 14 100 99.5 99.5 99.5 99.5 93.4 93.1 93.7 93.7 93.4 98.8 99.5 99.5
lpl0803A ORF 13 - 40.3 40.3 40.3 40.3 trans.c 100 98.2 98.2 96.6 41.8 40.3 40.3
lpg0765 ORF 12 100 98.6 98.7 98.6 98.6 - - - - - 98.7 98.6 trans.c
lpg0766 ORF 11 100 96.6 96.6 96.6 96.6 93.2 93.2 93.7 93.7 93.1 96.6 96.6 96.6
lpg0767 ORF 10 100 96.2 96.2 96.2 96.2 96.6 97.1 98.9 98.9 97 95.6 96.2 96.2
lpg0768 ORF 9 100 30.6 30.6 30.6 30.6 98.4 99 99 99 98.9 99.4 30.6 30.6
lpg0769 ORF 8 100 31 31 31 31 97.9 97.4 98.4 98.4 97.4 100 31 31
lpg0770 ORF 7 100 90.6 90.6 90.6 90.6 32 31.9 31.9 31.9 99.8 99.9 90.6 90.6
lpg0771 ORF 6 100 38.8 38.7 38.7 38.7 38.8 99.1 100 100 38.8 38.6 99.1 38.7
lpg0772 (wzm) ORF 5 100 100 100 100 100 100 100 100 100 100 100 100 100
lpg0773 (wzt) ORF 4 100 99 99.6 100 100 100 99.6 100 99.5 99 99.8 100 100
lpg0774 ORF 3 100 91.6 86.4 98.7 92.1 89 86.4 100 86.4 91.6 99.5 99.8 99.8
lpg0775a 100 - 100 - - - - - - - - -
lpg0776b 100 - - 100 - - - - - - - - -
lpg0777 (lag-1) 100 96.8 94.9 100 96.8 94.9 94.9 - 94.7 96.8 - - -
lpg0778 ORF 2 100 97.9 97.4 100 97.7 97.4 97.4 99.6 96.5 97.9 98.9 98.7 98.7
lpg0779 ORF 1 100 99.8 99.1 99.8 99.8 98.9 98.9 100 98.9 99.8 99.4 99.8 99.8

# Monoclonal antibody subgroup according to the ‘Dresden’ panel.

* Determined by UPGMA clustering method based on multiple sequence alignment.

A ORF 13 (lpg0764/lpg0764b/lpg0763) of Philadelphia 1 not displayed, ORF 13-A of strain Lens was used.

a Partial duplication of ORF 2 (lpg0778).

b, c Transposase; transposase disrupted.

Lag-1 of Görlitz 6543 has no functional start codon.

Underlined numbers indicate different clusters of corresponding ORF (see also Figure  2).

The highly conserved 15 kb region (ORF14 – ORF 28) is not completely shown and only reflected by WecA and GalE.

Petzold et al.

Petzold et al. BMC Microbiology 2013 13:198   doi:10.1186/1471-2180-13-198

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