WNV release is inhibited on expression of Tsg-5’ and Alix V domain. 293T cells were transfected with WNV-CPrME and Ren/Rep plasmids along with control pUC or the indicated cellular protein expression constructs. Virus release was determined using the (A) classical radioimmunoprecipitation technique and (B) the rapid ren-luc based assay. Data represent mean ± SD from 3 (A) or 4 (B) independent experiments.
Garg et al. BMC Microbiology 2013 13:197 doi:10.1186/1471-2180-13-197