Time course of antibody titer levels induced in mice immunized with PL10, PH10, PM10 and PBS. Four groups (PL10 coupled to KLH, PH10 coupled to KLH, PM10 coupled to KLH, and PBS), each comprising of ten adult female Balb/c mice (4–6weeks old), were immunized thrice with 2-week intervals using 100 μg of the PL10, PH10, PM10 or an equal volume PBS. PH10 ,PM10 and PBS were used as control. The mice were bled on week2, 4 and 6 via tail vein, and the anti-peptide antibody titer of mice sera was determined by ELISA. The antibody titer of PL10 was remarkably higher than that of the antisera from mice immunized with PH10, PM10 and PBS at each time point. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD); * P < 0.05 vs control groups (PH10, PM10 and PBS).
Luo et al. BMC Microbiology 2013 13:194 doi:10.1186/1471-2180-13-194