Deferred inhibition assay for bacteriocin production. Test strains were grown on mMRS and overlayered with Enterococcus faecalis FUA3141, which was as an indicator strain. Panel a, no addition of proteinase; panel b, addition of proteinase K adjacent to colonies of test strains. Arrows indicate the site of proteinase K application. The following test strains were used, 1, Ped. acidilactici FUA3138; 2, Ped. acidilactici FUA3072; 3, Ped. acidilactici FUA3140; 4, Lact. sakei FUA3089. Similar results were observed with Listeria innocua ATCC33090 used as an indicator strain (data not shown). The indicator strains of E. coli FUA1036, FUA1063 and FUA1064 were also used but no inhibition was observed (data not shown).
Wang et al. BMC Microbiology 2013 13:19 doi:10.1186/1471-2180-13-19