Additional file 5: Figure S5.

Examination of CHLA and PUG treatment on RSV cell-to-cell spread. HEp-2 cells were inoculated and infected with RSV for 1.5 h, washed with citrate buffer to remove excess surface bound virus, and covered with an overlay medium to prevent secondary infection. Initial virus plaques were allowed to form in the subsequent infections and CHLA, PUG, Heparin, and DMSO control were added to the overlay medium for an additional incubation time before analysis of viral plaque size by immune fluorescence microscopy at 48 h post-infection as described in Methods. Representative virus plaques/foci are shown after three independent experiments were performed. Scale bar indicates 100 μm.

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Lin et al. BMC Microbiology 2013 13:187   doi:10.1186/1471-2180-13-187