RecU-depleted cells show increased frequency of SpoIIIE-YFP foci. The figure shows SpoIIIE-YFP localization in recU inducible strain BCBRP002 incubated in the absence (A) or presence (B) of IPTG. SpoIIIE-YFP foci are present in 44% of BCBRP002 RecU-depleted cells in comparison with 10% of the cells of the same strain when expressing RecU. Panels from left to right show phase-contrast image, membrane labeled with FM 5–95, DNA stained with Hoechst 33342, SpoIIIE-YFP localization, and the overlay of the three fluorescence images showing the membrane in red, DNA in blue and SpoIIIE-YFP in yellow. Scale bars 1 μm.
Pereira et al. BMC Microbiology 2013 13:18 doi:10.1186/1471-2180-13-18