Analysis of variant and ancestral strain biofilm co-cultures.P. fluorescens variants and ancestral strain co-cultures were analyzed by the introduction of different colour AFPs. CLSM images were obtained on 96 h biofilms grown in the CBD. See ‘Materials and Methods’ for details of acquisition parameters. Multiple replicates were obtained for each biofilm co-culture and shown here are the best representative images. The images show a top-view 3D reconstruction of the biofilm along with a cross-section through the y-axis. Scale bars represent 40 μm. A, Controls showing that the two variants grow evenly together and the wildtype (CHA0) and ΔgacS (CHA19) also grow evenly distributed throughout the biofilm. B, Paired co-cultures of the CHA0 and CHA19 strains with either the SCV or WS. C, Triple co-cultures were done, where the SCV and WS were cultured together with either CHA0 or CHA19.
Workentine et al. BMC Microbiology 2013 13:175 doi:10.1186/1471-2180-13-175