Additional file 1.
PFGE analysis of environmental and clinical Legionella pneumophila strains. Legionella DNA samples were digested with SfiI restriction enzyme for 16 h at 50°C. Fragments of DNA were separated in a 0.8% agarose gel prepared and run in 0.5x Tris-borate-EDTA buffer (pH 8.3) in a contour-clamped homogeneous field apparatus with a constant voltage of 150 V. Runs were carried out with increasing pulse times (2 to 25 s) at 10°C for 11 h and increasing pulse times (35 to 60 s) at 10°C for 9 h.
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Chaabna et al. BMC Microbiology 2013 13:17 doi:10.1186/1471-2180-13-17