Open Access Highly Accessed Research article

The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

Pablo Rodrigo Hardoim15*, Rashid Nazir1, Angela Sessitsch2, Dana Elhottová3, Elisa Korenblum1, Leonard Simon van Overbeek4 and Jan Dirk van Elsas1

Author Affiliations

1 Department of Microbial Ecology, University of Groningen, Centre for Ecological and Evolutionary Studies, Nijenborgh 7, Groningen 9747AG, The Netherlands

2 AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria

3 Institute of Soil Biology, Biology Centre, ASCR, v.v.i., Na Sádkách 7, České Budějovice CZ 370 05, Czech Republic

4 Plant Research International, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands

5 Current address: Centre of Marine Science, University of Algarve, Faro 8005-139, Portugal

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BMC Microbiology 2013, 13:164  doi:10.1186/1471-2180-13-164

Published: 16 July 2013

Additional files

Additional file 2: Figure S2:

Maximum-likelihood tree based on rpoB gene sequences showing the phylogenetic position of Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. within the genus Enterobacter. A total of 45 nucleotide sequences (with 56 variable positions from a total of 495) were used, scoring the arithmetic means of log likelihood -3536.24. The nodes in terminal branches supported by ≥ 50% of the ML bootstrap analysis and homogeneous Bayesian (BI) posterior probabilities are shown. The tree is drawn to scale with bar indicating 0.06% substitutions per nucleotide position. Sequences from Pantoea genus were used as outgroup.

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Additional file 3: Table S1:

Fatty acid profiles of strains REICA_142T, REICA_084, REICA_191, REICA_082T, REICA_032, REICA_211 and type strains of closely related species of the genus Enterobacter measured by gas chromatography.

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Additional file 1: Figure S1:

Maximum-likelihood tree based on nearly complete 16S rRNA gene sequences showing the phylogenetic position of Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. within the genus Enterobacter. A total of 41 nucleotide sequences (with 131 variable positions from a total of 1125) were used, scoring the arithmetic means of log likelihood -3228. The nodes in terminal branches supported by ≥ 50% of the ML bootstrap analysis and homogeneous Bayesian (BI) posterior probabilities are shown. The tree is drawn to scale with bar indicating 0.05% substitutions per nucleotide position. Sequences from Pantoea genus were used as outgroup.

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Additional file 4: Figure S3:

Dendrogram derived from the fatty acid (FA) patterns showing the positions of Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. within the Enterobacteriaceae.

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