Table 4

Growth media used in this work
Medium Composition
RPMI 8.4 g L-1 RPMI 1640 (Sigma-Aldrich R1383), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH
YNB 6.7 g L-1 Yeast Nitrogene Base (Sigma Y1250), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH
YPD Sufficient iron medium: Yeast extract (10 g L-1) peptone (20 g L-1) dextrose (20 g L-1) (Sigma-Aldrich Y1375)
RIM Restricted iron medium; YPD + 200 μM bathophenantroline disulfunate (BPS) (Sigma 146617)

Kaba et al.

Kaba et al. BMC Microbiology 2013 13:16   doi:10.1186/1471-2180-13-16

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