Additional file 6.

Deletion of HOG1 had no influence on C. albicans growth in media with high iron concentrations. The WT (SC5314), the reference strain (DAY286), and the Δhog1 (JMR114) and Δpbs2 (JJH31) mutants were diluted in YPD each to ca. 0.5 · 106 cells ml-1 and further diluted in 1:10 steps. 5 μl of each cell suspension were dropped on RPMI agar plates containing 0 (RPMI), 1 or 30 μM FeCl3. Plates were incubated for 2 d at 30°C before pictures were taken. All plates were prepared in triplicates and one representative for each plate is shown.

Format: TIFF Size: 88KB Download file

Kaba et al. BMC Microbiology 2013 13:16   doi:10.1186/1471-2180-13-16