Additional file 4.

Effect of cycloheximide pre-incubation on iron induced flocculation. (A) Relative sedimentation rates of DAY286 cells treated with cycloheximide (CHX) C. albicans DAY286 was pre-treated either with 500 μg ml-1 CHX or MeOH in RPMI at 30°C for 15 min. Iron or water were subsequently added and cells were incubated at 30°C for 2 h. Sedimentation rates were determined as described in the experimental part. Means and standard deviations of three independent samples are shown (n = 3). ** denotes P ≤ 0.01 (student’s t-test). (B) Microscopic analysis of CHX or MeOH pre-treated cells (see A).

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Kaba et al. BMC Microbiology 2013 13:16   doi:10.1186/1471-2180-13-16