Figure 1.

Iron induced concentration dependent flocculation of C. albicans cells. (A) Microscopic analysis. C. albicans SC5314 (WT) was incubated with different FeCl3 concentrations (indicated at the top left hand of each sub panel) or with 30 μM FeSO4 in RPMI at 30°C for 2 h. (B) Relative sedimentation rates of WT cells. Flocculation of cells was triggered by 30 μM FeCl3 or 30 μM FeSO4 in RPMI and sedimentation rates were determined after incubation at 30°C for 2 h. Means and standard deviations of three independent samples are shown (n = 3). ** denotes P < 0.01 (student’s t-test). (C) Relative sedimentation rates of WT cells pre-cultured in the sufficient iron (YPD) or restricted iron medium (RIM) at 30°C for 3 h. Flocculation of cells was triggered by 30 μM FeCl3 in RPMI and sedimentation rates were determined after incubation at 30°C for 2 h. Means and standard deviations of three independent samples are shown (n = 3). *** denotes P < 0.001 (student’s t-test). (D) Microscopic analysis of cycloheximide (CHX) or MeOH pre-treated cells. C. albicans SC5314 was pre-treated either with 500 μg ml-1 CHX or MeOH in RPMI at 30°C for 15 min. Iron or water were subsequently added and cells were incubated at 30°C for 2 h.

Kaba et al. BMC Microbiology 2013 13:16   doi:10.1186/1471-2180-13-16
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