Open Access Methodology article

A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Ismawati Mohammad Amin1, Grace E Richmond3, Paromita Sen1, Tse Hsien Koh2, Laura JV Piddock3 and Kim Lee Chua1*

Author Affiliations

1 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore

2 Department of Pathology, Singapore General Hospital, Outram RoadSingapore169608

3 School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

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BMC Microbiology 2013, 13:158  doi:10.1186/1471-2180-13-158

Published: 13 July 2013

Additional files

Additional file 1: Table S1:

Description of primers used for PCR and DNA sequencing. Table S2. List of primers used for quantitative real-time PCR.

Format: DOCX Size: 17KB Download file

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