Table 2

Proteins found to be differentially regulated by σL, as determined by a proteomic comparison between L. monocytogenes 10403S ΔBCH and ΔBCHL
Proteina Fold change Δ BCH BCHL Description Gene name Role categoryb Sub-Role categoryb
Proteins with positive fold change (> 1.5) and p < 0.05 (indicating positive regulation by σL)
Lmo0096d,f 64.16 mannose-specific PTS system IIAB component ManL mptA Energy metabolism Pyruvate dehydrogenase
Amino acid biosynthesis Aromatic amino acid family
Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo2006g 3.41 acetolactate synthase catabolic alsS Amino acid biosynthesis Aspartate family
Amino acid biosynthesis Pyruvate family
Proteins with negative fold change (< -1.5) and p < 0.05 (indicating negative regulation by σL)
Lmo0027c,e −3.62 beta-glucoside-specific PTS system IIABC component lmo0027 Transport and binding proteins Carbohydrates, organic alcohols, and acids
Amino acid biosynthesis Aromatic amino acid family
Energy metabolism Pyruvate dehydrogenase
Lmo0130 −3.64 hypothetical protein lmo0130 Unclassified Role category not yet assigned
Lmo0178 −2.07 hypothetical protein lmo0178 Regulatory functions Other
Lmo0181 −3.25 multiple sugar transport system substrate-binding protein lmo0181 Transport and binding proteins Unknown substrate
Lmo0260 −1.68 hydrolase lmo0260 Hypothetical proteins Conserved
Lmo0278 −1.67 maltose/maltodextrin transport system ATP-binding protein lmo0278 Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo0319c,e −2.96 beta-glucosidase bglA Energy metabolism Sugars
Lmo0343 −3.94 transaldolase tal2 Energy metabolism Pentose phosphate pathway
Lmo0344 −4.69 short chain dehydrogenase lmo0344 Energy metabolism Biosynthesis and degradation of polysaccharides
Lmo0345 −6.04 ribose 5-phosphate isomerase B lmo0345 Energy metabolism Pentose phosphate pathway
Lmo0346 −2.74 triosephosphate isomerase tpiA2 Energy metabolism Glycolysis/gluconeogenesis
Lmo0348 −2.41 dihydroxyacetone kinase lmo0348 Fatty acid and phospholipid metabolism Biosynthesis
Energy metabolism Sugars
Lmo0391 −1.67 hypothetical protein lmo0391
Lmo0401 −2.16 alpha-mannosidase lmo0401 Unclassified Role category not yet assigned
Lmo0517e −3.21 phosphoglycerate mutase lmo0517 Energy metabolism Glycolysis/gluconeogenesis
Lmo0521 −2.23 6-phospho-beta-glucosidase lmo0521 Energy metabolism Sugars
Lmo0536 −1.97 6-phospho-beta-glucosidase lmo0536 Central intermediary metabolism Other
Lmo0574 −1.65 6-phospho-beta-glucosidase GmuD lmo0574 Central intermediary metabolism Other
Lmo0640 −1.78 oxidoreductase lmo0640 Energy metabolism Fermentation
Central intermediary metabolism Other
Energy metabolism Electron transport
Lmo0643 −2.61 transaldolase lmo0643 Energy metabolism Pentose phosphate pathway
Lmo0689 −1.71 chemotaxis protein CheV lmo0689 Cellular processes Chemotaxis and motility
Lmo0690 −2.44 flagellin flaA Cellular processes Chemotaxis and motility
Lmo0692 −1.66 chemotaxis protein CheA cheA Cellular processes Chemotaxis and motility
Lmo0813 −2.04 fructokinase lmo0813 Energy metabolism Sugars
Lmo0930 −1.88 hypothetical protein lmo0930 Unclassified Role category not yet assigned
Lmo1242 −1.59 hypothetical protein lmo1242 Hypothetical proteins Conserved
Lmo1254 −2.10 alpha-phosphotrehalase lmo1254 Energy metabolism Biosynthesis and degradation of polysaccharides
Lmo1348 −2.42 glycine cleavage system T protein gcvT Energy metabolism Amino acids and amines
Lmo1349 −2.68 glycine cleavage system P-protein gcvPA Energy metabolism Amino acids and amines
Central intermediary metabolism Other
Lmo1350e −2.11 glycine dehydrogenase subunit 2 gcvPB Central intermediary metabolism Other
Energy metabolism Amino acids and amines
Lmo1388e −2.02 ABC transport system tcsA Unclassified Role category not yet assigned
Lmo1389 −2.32 simple sugar transport system ATP-binding protein lmo1389 Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo1538e −1.89 glycerol kinase glpK Energy metabolism Other
Lmo1699 −1.92 Methyl-accepting chemotaxis protein lmo1699 Cellular processes Chemotaxis and motility
Lmo1730 −2.55 lactose/L-arabinose transport system substrate-binding protein lmo1730 Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo1791 −1.75 hypothetical protein lmo1791
Lmo1812 −1.70 L-serine dehydratase iron-sulfur-dependent alpha subunit lmo1812 Energy metabolism Amino acids and amines
Energy metabolism Glycolysis/gluconeogenesis
Lmo1856 −1.65 purine nucleoside phosphorylase deoD Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides
Lmo1860 −1.64 peptide-methionine (S)-S-oxide reductase msrA Protein fate Protein modification and repair
Lmo1877 −2.14 formate-tetrahydrofolate ligase fhs Amino acid biosynthesis Aspartate family
Protein synthesis tRNA aminoacylation
Amino acid biosynthesis Histidine family
Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis
Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A
Lmo1954e −1.97 phosphopentomutase deoB Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides
Lmo1993 −1.81 pyrimidine-nucleoside phosphorylase pdp Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides
Lmo2094 −28.99 hypothetical protein lmo2094 Energy metabolism Sugars
Lmo2097 −12.12 galactitol-specific PTS system IIB component lmo2097 Energy metabolism Pyruvate dehydrogenase
Amino acid biosynthesis Aromatic amino acid family
Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo2098 −3.96 galactitol-specific PTS system IIA component lmo2098 Energy metabolism Pyruvate dehydrogenase
Amino acid biosynthesis Aromatic amino acid family
Transport and binding proteins Carbohydrates, organic alcohols, and acids
Lmo2160 −2.37 sugar phosphate isomerase/epimerase lmo2160 Hypothetical proteins Conserved
Lmo2161 −2.58 hypothetical protein lmo2161 Hypothetical proteins Conserved
Lmo2362 −1.87 glutamate/gamma-aminobutyrate antiporter lmo2362 Transport and binding proteins Amino acids, peptides and amines
Lmo2425 −1.59 glycine cleavage system H protein gcvH Energy metabolism Amino acids and amines
Lmo2481 −1.52 pyrophosphatase PpaX ppaX Central intermediary metabolism Other
Lmo2529 −1.72 ATP synthase F1 beta subunit atpD2 Energy metabolism ATP-proton motive force interconversion
Lmo2648 −2.50 hypothetical protein lmo2648 Unclassified Role category not yet assigned
Lmo2664 −1.72 L-iditol 2-dehydrogenase lmo2664 Central intermediary metabolism Other
Energy metabolism Glycolysis/gluconeogenesis
Energy metabolism Electron transport
Energy metabolism TCA cycle
Energy metabolism Fermentation
Lmo2696 −2.68 dihydroxyacetone kinase L subunit lmo2696 Energy metabolism Sugars
Fatty acid and phospholipid metabolism Biosynthesis
Lmo2697 −3.10 dihydroxyacetone kinase lmo2697 Hypothetical proteins Conserved
Lmo2743 −2.71 transaldolase tal1 Energy metabolism Pentose phosphate pathway

aProtein names are based on the L. monocytogenes EGD-e locus.

bRole Categories and Sub-Role categories are based on JCVI classification [26].

cReported as negatively regulated by σL in Chaturongakul et al., 2011 [7].

dReported as downregulated in a rpoNL) mutant compared to wildtype L. monocytogenes EGD-e in Arous et al., 2004 [22].

eReported as upregulated in a rpoNL) mutant compared to wildtype L. monocytogenes EGD-e in Arous et al., 2004 [22].

fPreceded by a putative σL promoter;

cagaac ; -12 and -24 regions are underlined.

gPreceded by a putative σA promoter;

taattcttttgagtag ; -10 and -35 regions are underlined.

Mujahid et al.

Mujahid et al. BMC Microbiology 2013 13:156   doi:10.1186/1471-2180-13-156

Open Data