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Open Access Research article

Potential of bacteriophage ΦAB2 as an environmental biocontrol agent for the control of multidrug-resistant Acinetobacter baumannii

Li-Kuang Chen12, Yu-Lin Liu3, Anren Hu4, Kai-Chih Chang4, Nien-Tsung Lin5, Meng-Jiun Lai4 and Chun-Chieh Tseng3*

Author affiliations

1 Institute of Medical Sciences, Department of Laboratory Diagnostics, College of Medicine, Tzu Chi University, Hualien, Taiwan

2 Department of Laboratory Medicine, Clinical Pathology, Emerging Infectious Pathogen Research Laboratory, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

3 Department and Graduate Institute of Public Health, Tzu Chi University, Hualien, Taiwan

4 Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan

5 Master Program, Microbiology, Immunology, and Biochemistry, School of Medicine, Tzu Chi University, Hualien, Taiwan

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Citation and License

BMC Microbiology 2013, 13:154  doi:10.1186/1471-2180-13-154

Published: 8 July 2013

Abstract

Background

Multidrug-resistant Acinetobacter baumannii (MDRAB) is associated with nosocomial infections worldwide. To date, the use of a phage to prevent infections caused by MDRAB has not been demonstrated.

Results

The MDRAB-specific phage ϕAB2 was stable at 4°C and pH 7 in 0.5% chloroform solution, and showed a slight decrease in plaque-forming units (PFU)/ml of 0.3–0.9 log after 330 days of storage. The addition of ϕAB2 at a concentration of at least 105 PFU/ml to an A. baumannii M3237 suspension killed >99.9% of A. baumannii M3237 after 5 min, regardless of A. baumannii M3237 concentration (104, 105, or 106 colony-forming units (CFU)/ml). The addition of ϕAB2 at a concentration of 108 PFU/slide (>107 PFU/cm2) to glass slides containing A. baumannii M3237 at 104, 105, or 106 CFU/slide, significantly reduced bacterial numbers by 93%, 97%, and 99%, respectively. Thus, this concentration is recommended for decontamination of glass surfaces. Moreover, infusion of ϕAB2 into 10% glycerol exhibited strong anti-MDRAB activity (99.9% reduction), even after 90 days of storage. Treatment of a 10% paraffin oil-based lotion with ϕAB2 significantly reduced (99%) A. baumannii M3237 after 1 day of storage. However, ϕAB2 had no activity in the lotion after 1 month of storage.

Conclusions

Phages may be useful for reducing MDRAB contamination in liquid suspensions or on hard surfaces. Phages may also be inoculated into a solution to produce an antiseptic hand wash. However, the phage concentration and incubation time (the duration of phage contact with bacteria) should be carefully considered to reduce the risk of MDRAB contamination.

Keywords:
Bacteriophage; Biocontrol; MDRAB