Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Phenotypes and/or characteristics Reference or source
B. cenocepacia
WT Wild type strain H111, Genomovars III of the B. cepacia complex 23
WT(GGDEF) Wild type strain harboring the expression construct pLAFR3-GGDEF 14
WT(wspR) Wild type strain harboring the expression construct pMLS7-wspR This study
∆rpfFBc BDSF-minus mutant derived from H111 with rpfFBc being deleted 14
∆rpfFBc(EAL) Mutant ∆rpfFBc harboring the expression construct pLAFR3-EAL 14
∆rpfFBc(rocR) Mutant ∆rpfFBc harboring the expression construct pMLS7-rocR 14
∆rpfFBc(wspR) Mutant ∆rpfFBc harboring the expression construct pMLS7-wspR This study
∆rpfFBc (rpfFBc) Mutant ∆rpfFBc harboring the expression construct pMLS7-rpfFBc 14
∆rpfFBc (cepI) Mutant ∆rpfFBc harboring the expression construct pMLS7-cepI This study
∆rpfFBc (PcepI-lacZ) Mutant ∆rpfFBc harboring the expression construct PcepI-lacZ This study
∆rpfR Deletion mutant with rpfR being deleted 14
∆rpfR(rpfR) Mutant ∆rpfR harboring the expression construct pMLS7-rpfR 14
∆rpfR(rpfRAAL) Mutant ∆rpfR harboring the expression construct pMLS7-rpfRAAL This study
∆rpfR(rpfRGGAAF) Mutant ∆rpfR harboring the expression construct pMLS7-rpfRGGAAF This study
∆cepI Deletion mutant with cepI being deleted 23
∆cepI(rpfFBc) Mutant ∆cepI harboring the expression construct pMLS7-rpfFBc This study
∆rpfFBc∆cepI Double deletion mutant with rpfFBc and cepI being deleted This study
∆rpfR (PcepI-lacZ) Mutant ∆rpfR harboring the expression construct PcepI-lacZ This study
BCAM0227 (PcepI-lacZ) Insertional mutant of BCAM0227 harboring the expression construct PcepI-lacZ This study
E. coli
DH5α supE44 ∆lacU169(Φ80lacZ∆M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 λpir Laboratory collection
OP50 Food source of C. elegans 22, 34
Agrobacterium tumefaciens
CF11 AHL reporter strain Lab of Stephen K. Farrand
Plasmid
pMLS7-rpfFBc pMLS7 containing rpfFBc 12
pMLS7-rpfR pMLS7 containing rpfR 14
pMLS7-rpfRAAL pMLS7-rpfR harboring an E443A amino acid substitution This study
pMLS7-rpfRGGAAF pMLS7-rpfR harboring a D318A and E319A amino acid substitution This study
pMLS7-cepI pMLS7 containing cepI 13
pMLS7-wspR pMLS7 containing wspR This study
pMLS7-rocR pMLS7 containing rocR This study
pLAFR3-GGDEF pLAFR3 containing the encoding region of the GGDEF domain of RpfR 14
pLAFR3-EAL pLAFR3 containing the encoding region of the EAL domain of RpfR 14
PcepI-lacZ pME2-lacZ containing promoter of cepI This study

Deng et al.

Deng et al. BMC Microbiology 2013 13:148   doi:10.1186/1471-2180-13-148

Open Data